Frangioni Laboratory: Data Sharing

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Phase-Referencing Two Signal Generators (200 kHz–100 MHz)— Homodyne frequency-domain photon migration (FDPM) imaging requires two signal generators that are perfectly phase-aligned. Even in a master/slave configuration, most signal generators will not be phase-referenced at power-up. This PDF describes, in detail, how to phase-reference, i.e., find a zero phase difference between, two GPIB-controlled Aeroflex signal generators using inexpensive hardware and custom LabVIEW VIs. The software itself is downloadable as a self-installing executable (.exe) file, or if preferred, the original LabVIEW VI source code.

Selection and Construction of an RF-Modulatable (200 KHz—500 MHz) Laser Diode System for Frequency-Domain Photon Migration (FDPM)—This protocol describes the key elements that must be considered when selecting a laser diode, mount, TEC cooler, and driver for near-infrared fluorescence excitation during FDPM experiments. It details the construction of a completely functioning laser diode system and describes how to couple laser output to optical fibers. The cost of the system construction and the importance of laser safety are also discussed.

Fluorescence Emission Calculator—This interactive Excel spreadsheet is described in detail in the Lim et al. manuscript (see below). It will calculate the number of fluorescence emission photons measured at the surface of a living tissue for any quantum dot or organic fluorophore embedded at a defined depth within that tissue.
Lim YT, Kim S, Nakayama A, Stott NE, Bawendi MG, Frangioni JV. Selection of quantum dot wavelengths for biomedical assays and imaging. Mol Imaging. . Request reprint

Sortable Degenerate Codon Chooser for Phage Display Libraries—This interactive Excel spreadsheet is described in detail in the Voss et al. manuscript (see below). It assists with the design of degenerate codons used for peptide phage display and permits sorting based on any combination of desired amino acids.
Voss SD, De Grand AM, Romeo GR, Cantley LC, Frangioni JV. An integrated vector system for cellular studies of phage display-derived peptides. Anal Biochem. . Request reprint
Rapid Protein Quantitation from Any Buffer—This protocol describes a simple dot blot assay that will quantify protein concentration in any type of buffer. Even complex cellular lysates in 1X SDS-PAGE sample buffer can be quantified. It requires inexpensive reagents and takes approximately 20 to 30 minutes to complete.
Scaling Coverslips, Plates, and Flasks for Cell Culture—This handy 1-page chart, in an Excel spreadsheet, can be taped to a cell culture hood and provides all scaling factors needed to split cells from one container to another at any desired confluence. For example, to split cells from a 90% confluent 10-cm plate to a 12-well plate at 45% confluence, simply read across the chart, find the scaling factor of 14.56 (which would represent 90% confluence) and divide by 2 (=29.12). If the 10-cm plate were trypsinized into a final volume of 10 ml of medium, just add 10 / 29.12 = 343 µL to each well of the 12-well to achieve the desired 45% confluence. The chart also provides proper gassing conditions for DMEM and RPMI media, as well as a convenient "counting chart" that can be used to convert the number of cells seen under any microscope field to the total number of cells in the container.